Don E. Crabtree, 1912–1980

Glucose transport in crabtree-positive and crabtree-negative yeasts.

The kinetic parameters of glucose transport in four Crabtree-positive and four Crabtree-negative yeasts were determined. The organisms were grown in aerobic glucose-limited chemostats at a dilution rate of 0.1 h-1. The results show a clear correlation between the presence of high-affinity glucose transport systems and the absence of aerobic fermentation upon addition of excess glucose to steady...

The Crabtree effect: a review.

Relief of the Crabtree Effect by Arsenate1

Arsenate, an uncoupler of oxidative phosphorylation, relieved the Crabtree effect in Ehrlich-Lett réhyperdiploid ascites tumor cells. Respiration was measured with an oxygen electrode. High arsenale concentrations (30 to 60 ITIM)were needed to produce a 2-fold increase in the depressed respiratory rate. Arsenate concentrations below 10 HTMhad little or no effect. The relief by arsenale was rev...

An evolutionary perspective on the Crabtree effect

The capability to ferment sugars into ethanol is a key metabolic trait of yeasts. Crabtree-positive yeasts use fermentation even in the presence of oxygen, where they could, in principle, rely on the respiration pathway. This is surprising because fermentation has a much lower ATP yield than respiration (2 ATP vs. approximately 18 ATP per glucose). While genetic events in the evolution of the C...

Dogged Don

Like the movements of the MinD protein, rapid move-1965 to 1996, the period covered by the book, Don Metcalf was Head of the Cancer Research Unit and ments are occurring in the intellectual landscape of cell Assistant Director of the Institute, which was headed biology, and cell polarity is no exception. This volume during this period by Gus Nossal. The focus of Metcalf's is a snapshot of the f...